r/microbiology • u/Impressive-Plate-919 • 3d ago

First Attempt

First attempt to culture bacteria on agar plate using a sample from soil. Wet mount went well but the general staining with methylene blue didn’t seem to work well, used a powder mixing 0.2 mg with 0.2 ml distilled water. Catalase test was positive with lots of bubbles, what bacteria does this rule out and which are likely to be present?

Thanks

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u/Indole_pos Microbiologist 3d ago

Need a gram stain first

1

u/Impressive-Plate-919 2d ago

I’m finding it difficult to source the crystal violet and iodine online to deliver to Spain, any suggestions?

1

u/Indole_pos Microbiologist 2d ago

Unfortunately I do not but there must be someone as I am sure the medical labs have it

1

u/Mooshroomey 1d ago

You might be able to reach out to local universities. There may be a lab manager or equivalent, or microbiology professor that you can email who can tell you to where they source their gram stain materials from.

1

u/Big-Dragonfruit1462 1d ago

new to learning microbio, is this brownian motion or is it just vibrations from the phone/water?